Influence of surgical instrument identifiers on microbial contamination of scalpel handles after cleaning and sterilization

Authors

DOI:

https://doi.org/10.5327/Z1414-4425202429939%20

Keywords:

Dental instruments, Sterilization, Microbiology

Abstract

Objective: To evaluate the influence of different instrument identifiers on the microbial growth of critical devices after cleaning and sterilization steps. Method: Fifteen No. 3 scalpel handles were used as test specimens, divided into 3 groups (n=5), with each group consisting of an instrument without an identifier and four other instruments with the following identifiers: laser engraving, silicone ring, vinyl identifier with permanent adhesive and personalized adhesive label. After being submerged in the surgical aspirate, the instruments were processed in accordance with the good practice requirements of Resolution No. 15/2012 from the National Health Surveillance Agency (Anvisa). Microbial growth was evaluated at different times qualitatively, by the presence or absence of growth, and quantitatively for positive cases, by counting viable colony-forming units (log CFU/scalpel handle). Results: At some point, there was microbial growth on the instruments regardless of the type of identifier. However, there was no continuous and progressive contamination after repeating the cleaning and sterilization steps. Conclusion: The type of dental instrument identifier does not interfere with microbial growth as long as cleaning and sterilization standards are respected.

Author Biographies

Renan Pereira Barbosa, Universidade Federal de Juiz de Fora, Departamento de Clínica Odontológica – Juiz de Fora (MG), Brasil.

Cirurgião-dentista, pós-graduação, Universidade Federal de Juiz de Fora, Departamento de Clínica Odontológica – Juiz de Fora - Minas Gerais – Brasil

Mariana Simões de Oliveira, Universidade Federal de Juiz de Fora, Departamento de Clínica Odontológica – Juiz de Fora (MG), Brasil.

Cirurgião-dentista, mestrado, Universidade Federal de Juiz de Fora, Departamento de Clínica Odontológica – Juiz de Fora - Minas Gerais – Brasil

Marcelle Silva Alves de Paula, Universidade Federal de Juiz de Fora, Departamento de Ciências Farmacêuticas – Juiz de Fora (MG), Brasil.

Farmacêutica, doutorado, Universidade Federal de Juiz de Fora, Departamento de Ciências Farmacêuticas. Juiz de Fora - Minas Gerais – Brasil

Ana Carolina Morais Apolônio, Universidade Federal de Juiz de Fora, Departamento de Parasitologia, Microbiologia e Imunologia – Juiz de Fora (MG), Brasil.

Cirurgião-dentista, doutorado, Universidade Federal de Juiz de Fora, Departamento de Parasitologia, Microbiologia e Imunologia – Juiz de Fora - Minas Gerais – Brasil

Matheus Furtado de Carvalho, Universidade Federal de Juiz de Fora, Departamento de Clínica Odontológica – Juiz de Fora (MG), Brasil.

Cirurgião-dentista, doutorado, Universidade Federal de Juiz de Fora, Departamento de Clínica Odontológica – Juiz de Fora – Minas Gerais - Brasil

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Published

2024-08-09

How to Cite

Souza, L. B. de, Barbosa, R. P., Oliveira, M. S. de, Paula, M. S. A. de, Apolônio, A. C. M., & Carvalho, M. F. de. (2024). Influence of surgical instrument identifiers on microbial contamination of scalpel handles after cleaning and sterilization. Revista SOBECC, 29. https://doi.org/10.5327/Z1414-4425202429939

Issue

Vol. 29 (2024)

Section

ORIGINAL ARTICLES

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Copyright (c) 2024 Laura Bellini de Souza, Renan Pereira Barbosa, Mariana Simões de Oliveira, Marcelle Silva Alves de Paula, Ana Carolina Morais Apolônio, Matheus Furtado de Carvalho

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